Reference Library

A collection of various Microbes. This library helps defines and describes the various elements you may find in your water.
Indicator morphotype: Filamentous Morphotype
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Fungi morphotype

Diameter:
5.0-10.0 µm
Dimensions
N/A
Shape:
Variable
Septa:
Sometimes Visible
Sulfur Granules:
No
Sheath:
No
Branching:
No

Description

Very large

Filament Type 0581

Diameter:
.5-.8 µm
Dimensions
Shape:
N/A
Septa:
Usually not visible
Sulfur Granules:
No
Sheath:
No
Branching:
No

Description

Looks like Microthix but gram positive.

Filament Type 0211 Morphotype

Diameter:
.3-.4 µm
Dimensions
.4 x .6 µm
Shape:
OvalsRods
Septa:
Yes usually visible
Sulfur Granules:
No
Sheath:
No
Branching:
No

Description

Grows dispersed. “Thin and crooked”.

Actinomycetes-Mycolata

Diameter:
.8-1.2 µm
Dimensions
1.0 x 1.0-2.0 µm
Actinomycetes-Mycolata cropped from 1000x Neisser (1)
Actinomycetes-Mycolata cropped from 1000x Neisser (1)
Shape:
Variable
Septa:
Yes - most species Septa is visible
Sulfur Granules:
No
Sheath:
No
Branching:
No

Description

“True branching”. Almost all species in wastewater stain gram positive when healthy.

Microthrix filament morphotype

Diameter:
.5-.8 µm
Dimensions
N/A
Shape:
Individual cell not visible
Septa:
Yes- but very rarely seen septa.
Sulfur Granules:
No
Sheath:
No
Branching:
No

Description

Gram positive. Often has Neisser positive granules.

Type 1851 Filament Morphotype

Diameter:
.8-1 µm
Dimensions
0.8 x 1.4 µm
type 1851-like filaments cropped from 1000x phase contrast
type 1851-like filaments cropped from 1000x phase contrast
Shape:
Rectangles
Septa:
Yes - often difficult to see
Sulfur Granules:
No
Sheath:
Yes
Branching:
Yes

Description

Often forms "bundles/twisted ropes". Often has attached growth.

Filament Type 1863 Morphotype

Diameter:
.8-1.0 µm
Dimensions
.8-1.0 x 1.0-1.5 µm
Shape:
OvalsRods
Septa:
Yes - visible
Sulfur Granules:
No
Sheath:
No
Branching:
No

Description

Usually dispersed. “Chain of cells” appearance.

Filament Type 0092

Diameter:
0.6-1.2 µm
Dimensions
.8-1.0 x 1.0
type 0092 cropped from 1000x Neisser
type 0092 cropped from 1000x Neisser
Shape:
Rectangles
Septa:
Yes - Sometimes but not always Visible
Sulfur Granules:
No
Sheath:
Variable
Branching:
Variable

Description

Neisser positive. Need Neisser stain to ID. Often difficult to see at phase contrast. Common to not see individual cells.

Filament Type 0961

Diameter:
1.0-1.4 µm
Dimensions
1.0-1.4 x 2.0-4.0 µm
Shape:
SausageRods
Septa:
Yes - visible
Sulfur Granules:
No
Sheath:
No - not a true sheath
Branching:
No - not a true sheath

Description

Transparent/ "lighter" appearance.

Filament Type 0411 Morphotype

Diameter:
.8-1.2 µm
Dimensions
.8-1.2 x 2.0-5.0 µm
type 0411 cropped from 1000x phase contrast
type 0411 cropped from 1000x phase contrast
Shape:
SausageRods
Septa:
Yes - visible
Sulfur Granules:
No
Sheath:
No
Branching:
No

Description

"Elongated rods/ long sausage links"

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Ryan Hennessy Wastewater Microbiology
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