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Flexibacter

Diameter:

N/A

Diameter:

N/A
Flexibacter cropped from 1000x DIC scaled

Shape:

Rods

Septa:

N/A

Sulfur Granules:

N/A

Sheath:

N/A

Branching:

Description

Rod shaped. Grow outside of the floc (or sometimes near the floc edges) and correlated with organic acid substrate and/or microaerophilic conditions.

Spirochaetes

Diameter:

N/A

Diameter:

N/A
Spirochaetes cropped form 1000x DIC

Shape:

N/A

Septa:

N/A

Sulfur Granules:

N/A

Sheath:

N/A

Branching:

Description

“Cork-screw-like” and motile. Grow outside of the floc and correlated with organic acid substrate and/or microaerophilic conditions.

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Spirilla

Diameter:

N/A

Diameter:

N/A
spirillum cropped from 1000x phase contrast

Shape:

N/A

Septa:

N/A

Sulfur Granules:

N/A

Sheath:

N/A

Branching:

Description

Small and slightly curved structure. Grow outside of the floc and correlated with organic acid substrate and/or microaerophilic conditions.

Globular Zoogloea

Diameter:

N/A

Diameter:

N/A
Globular Zoogloea cropped from 1000x DIC scaled

Shape:

N/A

Septa:

N/A

Sulfur Granules:

N/A

Sheath:

N/A

Branching:

Description

Associated with organic acid substrate. Generally, occur when there are high bacterial growth rates in the Initial Contact Zone (first 15-30 minutes) of the aeration basin.

Bug of the month

Fingered Zoogloea

Diameter:

N/A

Diameter:

N/A
Fingered zoogloea cropped from 1000x phase

Shape:

N/A

Septa:

N/A

Sulfur Granules:

N/A

Sheath:

N/A

Branching:

Description

Associated with organic acid substrate. Generally, occur when there are high bacterial growth rates in the Initial Contact Zone (first 15-30 minutes) of the aeration basin.

Bug of the month

Filament Type 0581

Diameter:

.5-.8 µm

Diameter:

Type 0581

Shape:

N/A

Septa:

Usually not visible

Sulfur Granules:

N/A

Sheath:

No

Branching:

Description

Looks like Microthix but gram positive.

Filament Type 0211 Morphotype

Diameter:

.3-.4 µm

Diameter:

.4 x .6 µm
Type 0211

Shape:

Ovals, Rods

Septa:

Yes usually visible

Sulfur Granules:

N/A

Sheath:

No

Branching:

Description

Grows dispersed. “Thin and crooked”.

Filament Type 0092

Diameter:

0.6-1.2 µm

Diameter:

.8-1.0 x 1.0
type 0092 cropped from 1000x Neisser

Shape:

Rectangles

Septa:

Yes - Sometimes but not always Visible

Sulfur Granules:

N/A

Sheath:

Variable

Branching:

Description

Neisser positive. Need Neisser stain to ID. Often difficult to see at phase contrast. Common to not see individual cells.

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Filament Type 0961

Diameter:

1.0-1.4 µm

Diameter:

1.0-1.4 x 2.0-4.0 µm
Type 0961

Shape:

Sausage, Rods

Septa:

Yes - visible

Sulfur Granules:

N/A

Sheath:

No - not a true sheath

Branching:

Description

Transparent/ “lighter” appearance.

Filament Type 0411 Morphotype

Diameter:

.8-1.2 µm

Diameter:

.8-1.2 x 2.0-5.0 µm
type 0411 cropped from 1000x phase contrast

Shape:

Sausage, Rods

Septa:

Yes - visible

Sulfur Granules:

N/A

Sheath:

No

Branching:

Description

“Elongated rods/ long sausage links”

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Ryan Hennessy Wastewater Microbiology
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