Course for Wastewater Microbiology Textbook

wastewater microbiology book by Ryan hennessy
For a $50 course application fee online tests are available which may be completed at the end of each chapter. Tests consist of between 10-20 multiple choice and true or false answers and a 90% or higher score is needed to pass each test. A limit of 3 attempts per chapter test apply. Amazon estimates approximately 3-4 hours to read the textbook. Upon completion of the tests an invoice ($50) and a certificate of completion form will be sent to the registered user’s email address. The bonus of applying the certificate to CEU hours is ultimately the responsibility of the course attendees. CEU credits (3 hours) are in progress for the Wisconsin DNR and posting of the course in additional regions for CEUs will be shared as this knowledge becomes available.

Course Contents

Chapter 1

Aerobic Biological Treatment Processes Overview

•General Overview
•Mixed Liquor
•Critical Biomass Growth Condition Parameters

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Chapter 2

Common Problems Encountered in Biological Treatment Processes

  • Filamentous Bulking Defined
  • Diffuse Floc Structure versus Filaments Extending or Bridging Flocs Together
  • Slime Bulking Defined
  • Pin Floc Defined
  • Denitrification Defined
  • Dispersed Growth Defined
  • Low Nutrient Availability Defined
  • Foaming/Scum in Wastewater Treatment Processes
  • Microscopy Considerations for Diagnosing Foam Causes
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Chapter 3

Microscopy Fundamentals

  • Types of Microscopes Recommended
  • How Phase Contrast Microscopy Works
  • Alignment of the Phase Rings
  • Example Microscope Supplies and Vendors
  • Reference for Microscope Settings Based on Desired Application
  • Troubleshooting Common Microscope Problems
  • Microscope Cameras
  • Optional Additions
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Chapter 4

Low Power Microscopy Observation Criteria

  • Slide Preparation
  • Judging Floc Morphology
  • Dispersed Growth Characteristics
  • Higher Life Form Organisms
  • Ranking Filamentous Bacteria Abundance at 10x and 20X
  • Location of Filamentous Bacteria
  • Judging Filamentous Bacteria’s Impact on Overall Floc Structure
  • Ranking of Traditional Zoogloea Bacteria Morphotypes
  • Example of Low Power Magnification Bench Sheet
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Chapter 5

Higher Life Form Organisms

  • Flagellates
  • Naked Amoebae
  • Testate Amoebae
  • Free Swimming Ciliates
  • Stalked Ciliates
  • Rotifers
  • Nematodes
  • Water Bear
  • Gastrotrich
  • Others
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Chapter 6

High Power Microscopy Observation Techniques

  • The “Hard Smash”
  • Bringing a Sample into Focus
  • Steps to Identifying an Unknown Filament Type
  • Filament Morphotypes Identification Table
  • Filamentous Morphotypes and Commonly Associated Causes
  • Compatible Bench Sheet for Identification Table Usage
  • Gram and Neisser Staining
  • Additional High-Power Microscopy Morphotypes of Importance
  • Fluorescent Microscopy Viability Applications
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Chapter 7

Filamentous Bulking Control Strategies Based on Causes

  • Selective Chlorination for Filament Control
  • “Sludge Juggling” and High Flow Events
  • Common Engineering Modifications for Filamentous Bulking Control
  • Common Engineering Modification for Filamentous Bulking Control
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Chapter 8

Other Troubleshooting and Strategies

  • “Slime Bulking”
  • Pin Floc
  • Denitrification
  • Dispersed Growth
  • Foaming in Aerobic Biological Processes
  • Stress, Inhibition, and Toxicity
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Chapter 9

Microscopy Considerations for Additional Types of Wastewater Processes

  • Membrane Bio Reactors
  • Fixed Film Sequences
  • Lagoons and Aerated Stabilization Basins

Anaerobic Treatment Processes

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Chapter 10

DNA Applications in Wastewater

  • Changes for Earlier Literature and Reasons Why
  • Notable Limitations of Wastewater Microscopy
  • Notable Limitations of DNA Sequencing (16SrRNA)
  • qPCR Testing Functionality
  • The Functional Roles of Genera and Other Areas of Interest in Wastewater Treatment Processes
  • Genetics versus Microscopy: Confirmed and Hypothetical Relationships for Filament Types
  • Additional Candidates and Correlations for Non-Filamentous Morphotypes
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