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Flexibacter

Diameter:

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Diameter:

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Flexibacter cropped from 1000x DIC scaled

Shape:

Rods

Septa:

N/A

Sulfur Granules:

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Sheath:

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Branching:

Description

Rod shaped. Grow outside of the floc (or sometimes near the floc edges) and correlated with organic acid substrate and/or microaerophilic conditions.

Spirochaetes

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Diameter:

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Spirochaetes cropped form 1000x DIC

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Sulfur Granules:

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Sheath:

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Branching:

Description

“Cork-screw-like” and motile. Grow outside of the floc and correlated with organic acid substrate and/or microaerophilic conditions.

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Spirilla

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Diameter:

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spirillum cropped from 1000x phase contrast

Shape:

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Septa:

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Branching:

Description

Small and slightly curved structure. Grow outside of the floc and correlated with organic acid substrate and/or microaerophilic conditions.

PAO (Polyphosphate Accumulating Organism)

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Diameter:

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PAO (Polyphosphate Accumulating Organism) Neisser 1000x scaled

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Description

Viewed upon Neisser staining as deeply Neisser positive grouping of cells.

Bug of the Month

Yeast

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Yeast 1000x phase cropped

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Description

“Balloon-man”- budding. Occur at low pH values or may enter wastewater treatment processes through influent or side-streams.

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Globular Zoogloea

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Diameter:

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Globular Zoogloea cropped from 1000x DIC scaled

Shape:

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Septa:

N/A

Sulfur Granules:

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Sheath:

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Branching:

Description

Associated with organic acid substrate. Generally, occur when there are high bacterial growth rates in the Initial Contact Zone (first 15-30 minutes) of the aeration basin.

Bug of the month

Fingered Zoogloea

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Diameter:

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Fingered zoogloea cropped from 1000x phase

Shape:

N/A

Septa:

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Sulfur Granules:

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Sheath:

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Branching:

Description

Associated with organic acid substrate. Generally, occur when there are high bacterial growth rates in the Initial Contact Zone (first 15-30 minutes) of the aeration basin.

Bug of the month

Nitrosomonas

Diameter:

N/A

Diameter:

N/A
Nitrosomonas 1000x DIC Cropped

Shape:

Honeycomb Like

Septa:

N/A

Sulfur Granules:

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Sheath:

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Branching:

Description

“Honeycomb-like”. Generally found on the edges of the floc. Nitrifying bacteria. AOB (ammonia oxidizing bacteria).

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GAO (Glycogen Accumulating Organism)

Diameter:

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Diameter:

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Glycogen Accumulating Organism Cropped from 1000x Neisser

Shape:

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Septa:

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Branching:

Description

Viewed upon Neisser staining. Neisser positive “grape-like” structure. Often believed to compete with PAOs (polyphosphate accumulating organisms) for substrate in anaerobic processes.

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Hyphomicrobium

Diameter:

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Diameter:

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Hyphomicrobium Cropped from 1000x phase contrast

Shape:

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Septa:

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Sulfur Granules:

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Sheath:

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Branching:

Description

Thin stalk with tip. Correlated to C1 compounds (i.e., methanol).

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Ryan Hennessy Wastewater Microbiology
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